AG Schubert

Projekte

In der Forschung werden folgende Schwerpunkte verfolgt:
Untersuchung der

Vielfalt der Funktionen von Ionenkanälen, insbesondere von Kaliumkanälen, im Kreislaufsystem verschiedener Organe (organotypische Bestimmung)
der Plastizität der arteriellen Kontraktilitätsmechanismen unter physiologischen (postnatales Remodeling) und pathophysiologischen (Diabetes) Bedingungen und die Regulation dieser Prozesse

Regulation and function of vascular smooth muscle potassium channels

We have shown that BK channels are regulated by a number of signalling pathways, by PKG (Robertson et al., 1993;Gribkova et al., 2002;Gagov et al., 2022), by PKA (Schubert et al., 1996) – also in hypertension (Pyanova et al., 2024), by PKC (Schubert et al., 1999b;Mladenov et al., 2008) as well as intracellular pH (Petkova-Kirova et al., 2000;Schubert et al., 2001). In small arteries, these signalling pathways mediate the contribution of BK channels to NO- and iloprost-induced dilations (Climent et al. 2012;Iozzi et al. 2013;Sausbier et al., 2000;Sausbier et al., 2005;Schubert et al., 1997), to ghrelin- (Mladenov et al., 2008) and methoxamine-induced vasocontraction, where the latter was shown to increase with postnatal maturation (Mladenov et al., 2008; Shvetsova et al., 2019, Ma et al., 2020) as well as to the setting of the level of the myogenic tone (Wesselman et al., 1997;Schubert et al., 1999a; Ma et al., 2020). Interestingly, when the full range of vessel tone was explored, NO-induced dilations were shown to be limited by BK channels, only at high levels of vessel tone BK channels facilitate this dilation (Schmid et al., 2018). All these findings were obtained on different small arteries from rats and PKG- as well as BK channel-deficient mice. Some of these data have been summarized in a review article (Schubert & Nelson, 2001).

Further, we could demonstrate that inwardly rectifying potassium (Kir2) channels are activated by NO, where this effect contributes to the NO-induced dilation of small arteries (Schubert et al., 2004) and that the negative feedback regulation of vasocontraction by Kir2 channels decreases during postnatal maturation (Shvetsova et al., 2019).

Ongoing studies are focused on the biophysical and pharmacological properties of several voltage-operated potassium channels and their function in the cardiovascular system. It was found that Kv7 channels contribute to the vasodilating effect of factors released from periadventitial fat (Schleifenbaum et al. 2010; Zavaritskaya et al. 2013, Tsvetkov et al 2016), as well as CO (Zhang et al. 2021), lose their dominating role in the negative feedback regulation of vasocontraction during postnatal maturation (Shvetsova et al., 2019; Ma et al., 2020) and are limited in their role as negative feedback on vasoconstriction by BK channels (Ma et al., 2020). These channels share with BK channels the ability to mediate vasodilation by the novel K channels opener GoSlo (Zavaritskaya et al. 2020). Another Kv channel, Kv2.1, was identified to prevent vasomotion and safeguard myogenic reactivity (Här et al., 2023). Recently, it was shown that another class of K channels, TASK-1 channels, also lose their ability to mediate negative feedback on vasocontraction during postnatal maturation (Shvetsova et al., 2020) and are involved in pH-dependent tone regulation in renal arteries (Shvetsova et al., 2022).

Overviews of the tools to study Kv7 channels and of the role of different K channels in early postnatal maturation have been presented in reviews (Gollasch et al., 2018; Svetsova et al., 2021; Schubert et al., 2023).

Climent, B., Schubert, R., Stankevicius, E., Garcia-Sacristan, A., Simonsen, U., Rivera, L. (2012). Large conductance Ca2+-activated K+ channels modulate endothelial cell outward currents and nitric oxide release in the intact rat superior mesenteric artery. BBRC 417, 1007-1013.

Gollasch,M., Welsh,D.G., Schubert,R. (2018). Perivascular adipose tissue and the dynamic regulation of Kv7 and Kir channels: Implications for resistant hypertension. Microcirculation. 25: e12434

Gribkova, I. V., Schubert, R., & Serebriakov, V. N. (2002). [Study of NO action on calcium-activated potassium channel of the rat artery smooth muscle cells]. Ross.Fiziol.Zh.Im I.M.Sechenova 88, 1199-1205.

Iozzi, D., Schubert, R., Kalenchuk, V.U., Neri, A., Sgaragli, G., Fusi, F., Saponara, S. (2013). Quercetin relaxes rat tail main artery partly via a PKG-mediated stimulation of KCa1.1 channels. Acta Physiol. 208, 329-39.

Ma,D., Gaynullina,D.K., Schmidt,N., Mladenov,M., Schubert,R. (2020). The functional availability of arterial Kv7 channels is suppressed considerably by large-conductance calcium-activated potassium channels in 2- to 3-month old but not in 10- to 15-day old rats. Front Physiol 11: 597395

Mladenov, M. I., Hristov, K. L., Dimitriova, D. Z., Schubert, R., Lubomirov, L. T., Gjorgoski, I. K., Duridanova, D. B., Gagov, H. S. (2008). Ghrelin signalling in guinea-pig femoral artery smooth muscle cells. Acta Physiol (Oxf) 194, 195-206.

Petkova-Kirova, P., Gagov, H., Krien, U., Duridanova, D., Noack, T., Schubert, R. (2000). 4-aminopyridine affects rat arterial smooth muscle BK(Ca) currents by changing intracellular pH. Br.J.Pharmacol. 131, 1643-1650.

Robertson, B. E., Schubert, R., Hescheler, J., Nelson, M. T. (1993). cGMP-Dependent Protein Kinase Activates Ca-Activated K- Channels in Cerebral Artery Smooth Muscle Cells. Am.J.Physiol. 265, C299-C303.

Sausbier, M., Arntz, C., Bucurenciu, I., Zhao, H., Zhou, X. B., Sausbier, U., Feil, S., Kamm, S., Essin, K., Sailer, C. A., Abdullah, U., Krippeit-Drews, P., Feil, R., Hofmann, F., Knaus, H. G., Kenyon, C., Shipston, M. J., Storm, J. F., Neuhuber, W., Korth, M., Schubert, R., Gollasch, M., Ruth, P. (2005). Elevated blood pressure linked to primary hyperaldosteronism and impaired vasodilation in BK channel-deficient mice. Circulation 112, 60-68.

Sausbier, M., Schubert, R., Voigt, V., Hirneiss, C., Pfeifer, A., Korth, M., Kleppisch, T., Ruth, P., Hofmann, F. (2000). Mechanisms of NO/cGMP-dependent vasorelaxation. Circ.Res. 87, 825-830.

Schleifenbaum, J., Köhn, C., Voblova, N., Dubrovska, G., Zavarirskaya, O., Gloe, T., Crean, C.S., Luft, F.C., Huang, Y., Schubert, R., Gollasch, M. (2010). Systemic peripheral artery relaxation by KCNQ channel openers and hydrogen sulfide. J Hypertens. 28, 1875-82.

Schmid,J., Müller,B., Heppeler,D., Gaynullina,D., Kassmann,M., Gagov,H., Mladenov,M., Gollasch,M., Schubert,R. (2018). The Unexpected Role of Calcium-Activated Potassium Channels: Limitation of NO-Induced Arterial Relaxation. J Am Heart Assoc. 7: e007808

Schubert, R., Krien, U., Gagov, H. (2001). Protons inhibit the BK(Ca) channel of rat small artery smooth muscle cells. J.Vasc.Res. 38, 30-38.

Schubert, R., Krien, U., Wulfsen, I., Schiemann, D., Lehmann, G., Ulfig, N., Veh, R. W., Schwarz, J. R., Gagov, H. (2004). Nitric oxide donor sodium nitroprusside dilates rat small arteries by activation of inward rectifier potassium channels. Hypertension 43, 891-896.

Schubert, R., Lehmann, G., Serebryakov, V. N., Mewes, H., Hopp, H.-H. (1999a). cAMP-dependent protein kinase is in an active state in rat small arteries possessing a myogenic tone. Am.J.Physiol. 277, H1145-H1155.

Schubert, R. Nelson, M. T. (2001). Protein kinases: tuners of the BKCa channel in smooth muscle. Trends Pharmacol.Sci. 22, 505-512.

Schubert, R., Noack, T., Serebryakov, V. N. (1999b). Protein kinase C reduces the KCa current of rat tail artery smooth muscle cells. Am.J.Physiol. 276, C648-C658.

Schubert, R., Serebryakov, V. N., Engel, H., Hopp, H.-H. (1996). Iloprost activates KCa channels of vascular smooth muscle cells: role of cAMP-dependent protein kinase. Am.J.Physiol. 271, C1203-C1211.

Schubert, R., Serebryakov, V. N., Mewes, H., Hopp, H.-H. (1997). Iloprost dilates rat small arteries: role of KATP- and KCa-channel activation by cAMP-dependent protein kinase. Am.J.Physiol. 272, H1147-H1156.

Shvetsova,A.A., Gaynullina,D.K., Tarasova,O.S., Schubert,R. (2019). Negative feedback regulation of vasocontraction by potassium channels in 10-to 15-day-old rats: Dominating role of K(v)7 channels Acta Physiol 225: e13176

Shvetsova,A., Gaynullina,D.K., Schmidt,N., Bugert,P., Lukoshkova,E.V., Tarasova,O.S., Schubert,R. (2020). TASK‐1 channel blockade by AVE1231 increases vasocontractile responses and BP in 1‐ to 2‐week‐old but not adult rats. Br. J. Pharmacol. 177:c5148-5162

Shvetsova,A.A., Gaynullina,D.K., Tarasova,O.S., Schubert,R. (2021). Remodeling of arterial tone regulation in postnatal development: focus on smooth muscle cell potassium channels. International Journal of Molecular Sciences 22: 5413

Tsvetkov, D., Tano, J.Y., Kassmann, M., Wang, N., Schubert, R., Gollasch, M. (2016). The Role of DPO-1 and XE991-Sensitive Potassium Channels in Perivascular Adipose Tissue-Mediated Regulation of Vascular Tone. Front Physiol. 7, 335

Wesselman, J. P. M., Schubert, R., VanBavel, E., Nilsson, H., Mulvany, M. J. (1997). KCa-channel blockade prevents sustained pressure-induced depolarization in rat mesenteric small arteries. Am.J.Physiol. 272, H2241-H2249.

Zavaritskaya, O., Zhuravleva, N., Schleifenbaum, J., Gloe, T., Devermann, L., Kluge, R., Mladenov, M., Frey, M., Gagov, H., Fésüs, G., Gollasch, M., Schubert, R. (2013). Role of KCNQ Channels in Skeletal Muscle Arteries and Periadventitial Vascular Dysfunction. Hypertension 61, 151-59.

Zavaritskaya,O., Dudem,S., Ma,D., Rabab,K.E., Albrecht,S., Tsvetkov,D., Kassmann,M., Thornbury,K., Mladenov,M., Kammermeier,C., Sergeant,G., Mullins,N., Wouappi,O., Wurm,H., Kannt,A., Gollasch,M., Hollywood,M.A., Schubert,R. (2020). Vasodilation of rat skeletal muscle arteries by the novel BK channel opener GoSlo is mediated by the simultaneous activation of BK and Kv7 channels. Br. J. Pharmacol. 177: 1164-1186

Zhang,D., Krause,B.M., Schmalz,H.G., Wohlfart,P., Yard,B.A., Schubert,R. (2021). ET-CORM Mediated Vasorelaxation of Small Mesenteric Arteries: Involvement of Kv7 Potassium Channels. Front Pharmacol. 6, 702392

Shvetsova,A.A., Lazarenko,V.S., Gaynullina,D.K., Tarasova,O.S., Schubert,R. (2022) TWIK-Related Acid-Sensitive Potassium Channels (TASK-1) Emerge as Contributors to Tone Regulation in Renal Arteries at Alkaline pH. Front. Physiol. 13:895863.

Gagov,H., Gribkova,I.V., Serebryakov,V.N., Schubert,R. (2022) Sodium Nitroprusside-Induced Activation of Vascular Smooth Muscle BK Channels Is Mediated by PKG Rather Than by a Direct Interaction with NO. International Journal of Molecular Sciences 23: 2798

Här,K., Lysenko,N.N., Dimitrova,D., Schlüter,T., Zavaritskaya,O., Kamkin,A.G., Mladenov,M., Grisk,O., Köhler,R., Gagov,H., Schubert,R. (2023) Kv2.1 Channels Prevent Vasomotion and Safeguard Myogenic Reactivity in Rat Small Superior Cerebellar Arteries. Cells 12: 1989

Schubert,R., Gaynullina,D.K., Shvetsova,A.A., Tarasova,O.S. (2023) Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary
techniques. Front. Physiol. 14: 1176748.

Pyanova,A., Serebryakov,V.N., Gagov,H., Mladenov,M., Schubert,R. (2024) BK Channels in Tail Artery Vascular Smooth Muscle Cells of Normotensive (WKY) and Hypertensive (SHR) Rats Possess Similar Calcium Sensitivity but Different Responses to the Vasodilator Iloprost. International Journal of Molecular Sciences 25: 7140

Functional remodeling of vascular smooth muscle during early postnatal development

Our studies showed that the signaling mechanisms governing vascular tone regulation possess a high degree of plasticity. Thus, with postnatal maturation (i) the anticontractile effect of the endothelium decreases (Gaynullina et al. 2013), (ii) sympathetic nerves reduce arterial smooth muscle Ca2+-sensitivity (Puzdrova et al. 2014), (iii) mRNA and miRNA expression changes in a correlated manner (Gaynullina et al. 2015), (iv) Rho-kinase-mediated calcium sensitivity decreases (Mochalov et al., 2018), (v) Kv1, Kv7 and Kir2-channel mediated negative feedback of vasocontraction is reduced, in the case of Kv7 channel in part due to an increasing role of BK channels (Shvetsova et al., 2019; Ma et al. 2020), and (vi) the TASK-1 channel mediated negative feedback of vasocontraction is lost (Shvetsova et al., 2020). Of note, an altered expression of signaling elements during maturation does not always translate into an altered functional role, as shown with MAP kinases (Gaynullina et al., 2021).

These data have been summarized in three reviews (Gaynullina et al., 2019; Shvetsova et al., 2021; Gaynullina et al., 2022).

Functional remodeling of vascular smooth muscle during early postnatal development *© Universität Augsburg*

Gaynullina, D., Dweep, H., Gloe, T., Tarasova, O.S., Sticht, C., Gretz, N., Schubert, R. (2015). Alteration of mRNA and microRNA expression profiles in rat muscular type vasculature in early postnatal development. Sci Rep. 5, 11106 doi: 10.1038/srep11106

Gaynullina, D., Lubomirov, L.T., Sofronova, S.I., Kalenchuk, V.U., Gloe, T., Pfitzer, G., Tarasova, O.S., Schubert, R. (2013). Functional remodelling of arterial endothelium during early postnatal development in rats. Cardiovasc. Res. 99, 612-21.

Gaynullina,D.K., Schubert,R., Tarasova,O.S. (2019). Changes in Endothelial Nitric Oxide Production in Systemic Vessels during Early Ontogenesis. A Key Mechanism for the Perinatal Adaptation of the Circulatory System Int. J. Mol. Sci. 20: 1421

Gaynullina,D.K., Kudryashova,T.V., Vorotnikov,A.V., Schubert,R., Tarasova,O.S. (2021). MAPKs are highly abundant but do not contribute to α1-adrenergic contraction of rat saphenous arteries in the early postnatal period. International Journal of Molecular Sciences 22: 6037

Mochalov,S.V., Tarasova,N.V., Kudryashova,T.V., Gaynullina,D.K., Kalenchuk,V.U., Borovik,A.S., Vorotnikov,A.V., Tarasova,O.S., Schubert,R. (2018). Higher Ca2+-sensitivity of arterial contraction in 1-week-old rats is due to a greater Rho-kinase activity. Acta Physiol 223: e13044

Puzdrova, V.A., Kudryashova, T.V., Gaynullina, D.K., Mochalov, S.V., Aalkjaer, C., Nilsson, H., Vorotnikov, A.V., Schubert, R., Tarasova, O.S. (2014). Trophic action of sympathetic nerves reduces arterial smooth muscle Ca2+-sensitivity during early postnatal development in rats. Acta.Physiol. 212, 128-141

Shvetsova,A.A., Gaynullina,D.K., Tarasova,O.S., Schubert,R. (2021) Remodeling of arterial tone regulation in postnatal development: focus on smooth muscle cell potassium channels. International Journal of Molecular Sciences 22: 5413

Gaynullina,D.K., Tarasova,O.S., Shvetsova,A.A., Borzykh,A.A., Schubert,R. (2022) The Effects of Acidosis on eNOS in the Systemic Vasculature: A Focus on Early Postnatal Ontogenesis. International Journal of Molecular Sciences 23: 5987

Mechanotransduction mechanisms in vascular smooth muscle cells

We could demonstrate that PKA (Schubert et al., 1999), Rho-kinase (Schubert et al., 2002) and BK as well as Kv7 channels (Sausbier et al., 2005, Ma et al. 2020) contribute to the setting of the level of small artery myogenic tone. Of note, Kv7 channels are limited in their negative feedback on myogenic tone by BK channels and vice versa (Ma et al., 2020). In addition, it was demonstrated that the myogenic response is mediated by Rho-kinase (Schubert et al., 2002) and modulated by neuronal influences (Anschutz & Schubert, 2005). The mechanisms of the myogenic response have been discussed in 4 review articles (Schubert & Mulvany, 1999;Schubert & Brayden, 2005;Schubert et al., 2008;Lidington et al. 2013).

We have been participating in a cooperative study showing that endothelial TRPV4 channels are involved in flow-induced vessel dilations (Kohler et al., 2006). A Kv channel, Kv2.1, was identified to prevent vasomotion and safeguard myogenic reactivity (Här et al., 2023).

Mechanotransduction mechanisms in vascular smooth muscle cells

Anschutz, S. Schubert, R. (2005). Modulation of the myogenic response by neurogenic influences in rat small arteries. Br.J.Pharmacol. 146, 226-233.

Kohler, R., Heyken, W. T., Heinau, P., Schubert, R., Si, H., Kacik, M., Busch, C., Grgic, I., Maier, T., Hoyer, J. (2006). Evidence for a functional role of endothelial transient receptor potential V4 in shear stress-induced vasodilatation. Arterioscler.Thromb.Vasc.Biol. 26, 1495-1502.

Lidington, D., Schubert, R., Bolz, S.S. (2013). Capitalizing on diversity: an integrative approach towards the multiplicity of cellular mechanisms underlying myogenic responsiveness. Cardiovasc. Res. 97, 404-12.

Schubert, R. Brayden, J. E. (2005). Stretch-activated cation channels and the myogenic response of small arteries. In Mechanosensitivity in Cells and Tissues pp. 402-417.

Schubert, R., Kalentchuk, V. U., Krien, U. (2002). Rho kinase inhibition partly weakens myogenic reactivity in rat small arteries by changing calcium sensitivity. Am.J.Physiol Heart Circ.Physiol 283, H2288-H2295.

Schubert, R., Lehmann, G., Serebryakov, V. N., Mewes, H., Hopp, H.-H. (1999). cAMP-dependent protein kinase is in an active state in rat small arteries possessing a myogenic tone. Am.J.Physiol. 277, H1145-H1155.

Schubert, R., Lidington, D., Bolz, S. S. (2008). The emerging role of Ca2+ sensitivity regulation in promoting myogenic vasoconstriction. Cardiovasc.Res. 77, 8-18.

Schubert, R. Mulvany, M. J. (1999). The myogenic response: established facts and attractive hypotheses. Clin.Sci. 96, 313-326.

Mechanisms of calcium sensitivity regulation

In our experiments we have demonstrated that Rho-kinase is contributing to the myogenic response by increasing the calcium sensitivity of the contractile apparatus (Schubert et al., 2002).

In addition, we have shown that the dilation induced by urocortin is caused by a PKA-mediated alteration of the calcium sensitivity (Lubomirov et al., 2001), associated with a dephosphorylation of MYPT1 and an activation of MLCP (Lubomirov et al., 2006a;Lubomirov et al., 2006b;Pfitzer et al., 2006). Further, we showed that Rho-kinase-mediated calcium sensitivity decreases with maturation (Mochalov et al., 2018).

Of note, for Src kinase, also known to affect calcium sensitivity, we discovered a major role in calcium-dependent pathways of contractility regulation (Zavaritskaya et al., 2017) suggesting that kinases have the potential to differentially affect calcium-sensitivity as well as calcium-dependent pathways.
Our data further demonstrate that sympathetic nerves reduce arterial smooth muscle Ca2+-sensitivity during postnatal development (Puzdrova et al. 2014).

We have been participating in cooperative studies showing that adenosine is increasing the calcium sensitivity of the contractile apparatus via p38 MAP-kinase (Martinka et al., 2008) and that expression of a leucine zipper positive splice variant of MYPT1 results in a higher NO/cGMP/PKG sensitivity of cerebral arteries (Lubomirov et al., 2018).

Mechanisms of calcium sensitivity regulation

Lubomirov, L., Gagov, H., Petkova-Kirova, P., Duridanova, D., Kalentchuk, V. U., Schubert, R. (2001). Urocortin relaxes rat tail arteries by a PKA-mediated reduction of the sensitivity of the contractile apparatus for calcium. Br.J.Pharmacol. 134, 1564-1570.

Lubomirov, L. T., Reimann, K., Metzler, D., Hasse, V., Stehle, R., Ito, M., Hartshorne, D. J., Gagov, H., Pfitzer, G., Schubert, R. (2006a). Urocortin-induced decrease in Ca2+ sensitivity of contraction in mouse tail arteries is attributable to cAMP-dependent dephosphorylation of MYPT1 and activation of myosin light chain phosphatase. Circ.Res. 98, 1159-1167.

Lubomirov, L. T., Schubert, R., Gagov, H. S., Duridanova, D. B., Pfitzer, G. (2006b). [Urocortin decreases phosphorylation of MYPT1 and increases the myosin phosphatase activity via elevation of the intracellular level of cAMP]. Biofizika 51, 773-780.

Lubomirov,L.T., Papadopoulos,S., Filipova,D., Baransi,S., Todorović,D., Lake,P., Metzler,D., Hilsdorf,S., Schubert,R., Schroeter,M.M., Pfitzer,G. (2018). The involvement of phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and MYPT1 isoform expression in NO/cGMP mediated differential vasoregulation of cerebral arteries compared to systemic arteries. Acta Physiol 224: e13079

Martinka, P., Lai, E. Y., Fahling, M., Jankowski, V., Jankowski, J., Schubert, R., Gaestel, M., Persson, A. E., Persson, P. B., Patzak, A. (2008). Adenosine increases calcium sensitivity via receptor-independent activation of the p38/MK2 pathway in mesenteric arteries. Acta Physiol (Oxf) 193, 37-46.

Pfitzer, G., Lubomirov, L. T., Reimann, K., Gagov, H., Schubert, R. (2006). Regulation of the crossbridge cycle in vascular smooth muscle by cAMP signalling. J.Muscle Res.Cell Motil. 27, 445-454.

Zavaritskaya,O., Lubomirov,L.T., Altay,S., Schubert,R. (2017) Src tyrosine kinases contribute to serotonin-mediated contraction by regulating calcium-dependent pathways in rat skeletal muscle arteries. Pflugers Arch. 469: 767-777

Publikationen

2024 | 2023 | 2022 | 2021 | 2020 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2002 | 2001 | 2000 | 1999 | 1998 | 1997 | 1996

2024

Pyanova Anastasia, Serebryakov Vladimir N., Gagov Hristo, Mladenov Mitko, Schubert Rudolf. BK channels in tail artery vascular smooth muscle cells of normotensive (WKY) and hypertensive (SHR) rats possess similar calcium sensitivity but different responses to the vasodilator iloprost. International Journal of Molecular Sciences 2024;25(13):7140. https://doi.org/10.3390/ijms25137140
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Orth Tobias, Pyanova Anastasia, Lux Simon, Kaiser Peter, Reinheimer Isabel, Løgstrup Nielsen Daniel, Khalid Josef Ali, Rognant Salomé, Jepps Thomas A., Matchkov Vladimir V., Schubert Rudolf. Vascular smooth muscle BK channels limit ouabain‐induced vasocontraction: dual role of the Na/K‐ATPase as a hub for Src‐kinase and the Na/Ca‐exchanger. The FASEB Journal 2024;38(17):e70046. https://doi.org/10.1096/fj.202400628rr
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2023

Sazdova Iliyana, Keremidarska-Markova Milena, Dimitrova Daniela, Mitrokhin Vadim, Kamkin Andre, Hadzi-Petrushev Nikola, Bogdanov Jane, Schubert Rudolf, Gagov Hristo, Avtanski Dimiter, Mladenov Mitko. Anticarcinogenic potency of EF24: an overview of its pharmacokinetics, efficacy, mechanism of action, and nanoformulation for drug delivery. Cancers 2023;15(22):5478. https://doi.org/10.3390/cancers15225478
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Schubert Rudolf, Shvetsova Anastasia A., Gaynullina Dina K., Tarasova Olga S.. Functional remodeling of arterial tone regulation in postnatal development: switch of the leading potassium channels from less to more calcium-dependent channel types [Abstract]. Journal of Vascular Research 2023;60(Supplement 1):36. https://doi.org/10.1159/000533846
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Drexler Stephan, Cai Chen, Hartmann Anna-Lena, Moch Denise, Gaitantzi Haristi, Ney Theresa, Kraemer Malin, Chu Yuan, Zheng Yuwei, Rahbari Mohammad, Treffs Annalena, Reiser Alena, Lenoir Bénédicte, Valous Nektarios A., Jäger Dirk, Birgin Emrullah, Sawant Tejas A., Li Qi, Xu Keshu, Dong Lingyue, Otto Mirko, Itzel Timo, Teufel Andreas, Gretz Norbert, Hawinkels Lukas J. A. C., Sánchez Aránzazu, Herrera Blanca, Schubert Rudolf, Moshage Han, Reissfelder Christoph, Ebert Matthias P. A., Rahbari Nuh N., Breitkopf-Heinlein Katja. Intestinal BMP-9 locally upregulates FGF19 and is down-regulated in obese patients with diabetes. Molecular and Cellular Endocrinology 2023;570:111934. https://doi.org/10.1016/j.mce.2023.111934
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Här Kristina, Lysenko Natalia N., Dimitrova Daniela, Schlüter Torsten, Zavaritskaya Olga, Kamkin Andrej G., Mladenov Mitko, Grisk Olaf, Köhler Ralf, Gagov Hristo, Schubert Rudolf. Kv2.1 channels prevent vasomotion and safeguard myogenic reactivity in rat small superior cerebellar arteries. Cells 2023;12(15):1989. https://doi.org/10.3390/cells12151989
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Schubert Rudolf, Gaynullina Dina, Shvetsova Anastasia, Tarasova Olga S.. Myography of isolated blood vessels: considerations for experimental design and combination with supplementary techniques. Frontiers in Physiology 2023;14:1176748. https://doi.org/10.3389/fphys.2023.1176748
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2022

Schubert Rudolf, Zhang D., Krause B., Schmalz H. G., Wohlfahrt P., Yard B.. CO-releasing molecules relax rat small mesenteric arteries: contribution of Kv7 channels [Abstract]. Acta Physiologica 2022;236(S725):855. https://doi.org/10.1111/apha.13877
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Sazdova Iliyana, Keremidarska-Markova Milena, Chichova Mariela, Uzunov Blagoy, Nikolaev Georgi, Mladenov Mitko, Schubert Rudolf, Stoyneva-Gärtner Maya, Gagov Hristo S.. Review of cyanotoxicity studies based on cell cultures. Journal of Toxicology 2022;2022:5647178. https://doi.org/10.1155/2022/5647178
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Gagov Hristo, Gribkova Irina, Serebryakov Vladimir, Schubert Rudolf. Sodium nitroprusside-induced activation of vascular smooth muscle BK channels is mediated by PKG rather than by a direct interaction with NO. International Journal of Molecular Sciences 2022;23(5):2798. https://doi.org/10.3390/ijms23052798
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Shvetsova Anastasia A., Lazarenko Varvara S., Gaynullina Dina K., Tarasova Olga S., Schubert Rudolf. TWIK-related acid-sensitive potassium channels (TASK-1) emerge as contributors to tone regulation in renal arteries at alkaline pH. Frontiers in Physiology 2022;13:895863. https://doi.org/10.3389/fphys.2022.895863
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Gaynullina Dina K., Tarasova Olga S., Shvetsova Anastasia A., Borzykh Anna A., Schubert Rudolf. The effects of acidosis on eNOS in the systemic vasculature: a focus on early postnatal ontogenesis. International Journal of Molecular Sciences 2022;23(11):5987. https://doi.org/10.3390/ijms23115987
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2021

Li Y., Faiz A., Moshage H., Schubert Rudolf, Schilling L., Kamps J. A.. Comparative transcriptome analysis of inner blood-retinal barrier and blood–brain barrier in rats. Scientific Reports 2021;11(1):12151. https://doi.org/10.1038/s41598-021-91584-7
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Zhang Danfeng, Krause Bernhard M., Schmalz Hans-Günther, Wohlfart Paulus, Yard Benito A., Schubert Rudolf. ET-CORM mediated vasorelaxation of small mesenteric arteries: involvement of Kv7 potassium channels. Frontiers in Pharmacology 2021;12:702392. https://doi.org/10.3389/fphar.2021.702392
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Bothe Tomas L., Patzak Andreas, Schubert Rudolf, Pilz Niklas. Getting it right matters! Covid‐19 pandemic analogies to everyday life in medical sciences. Acta Physiologica 2021;233(1):e13714. https://doi.org/10.1111/apha.13714
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Gaynullina Dina K., Kudryashova Tatiana V., Vorotnikov Alexander V., Schubert Rudolf, Tarasova Olga S.. MAPKs are highly abundant but do not contribute to α1-adrenergic contraction of rat saphenous arteries in the early postnatal period. International Journal of Molecular Sciences 2021;22(11):6037. https://doi.org/10.3390/ijms22116037
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Shvetsova Anastasia A., Gaynullina Dina K., Tarasova Olga S., Schubert Rudolf. Remodeling of arterial tone regulation in postnatal development: focus on smooth muscle cell potassium channels. International Journal of Molecular Sciences 2021;22(11):5413. https://doi.org/10.3390/ijms22115413
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Wennysia I. C., Zhao L., Schomber T., Braun D., Golz S., Summer H., Benardeau A., Lai E. Y., Lichtenberger F.-B., Schubert Rudolf, Persson P. B., Xu M. Z., Patzak A.. Role of soluble guanylyl cyclase in renal afferent and efferent arterioles. American Journal of Physiology-Renal Physiology 2021;320(2):F193-F202. https://doi.org/10.1152/ajprenal.00272.2020
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2020

Stamenkovska Mimoza, Thaçi Qendrim, Hadzi‐Petrushev Nikola, Angelovski Marija, Bogdanov Jane, Reçica Shkëlzen, Kryeziu Islam, Gagov Hristo, Mitrokhin Vadim, Kamkin Andre, Schubert Rudolf, Mladenov Mitko, Sopi Ramadan B.. Curcumin analogs (B2BrBC and C66) supplementation attenuates airway hyperreactivity and promote airway relaxation in neonatal rats exposed to hyperoxia. Physiological Reports 2020;8(16):e14555. https://doi.org/10.14814/phy2.14555
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Shvetsova Anastasia A., Gaynullina Dina K., Schmidt Nadine, Bugert Peter, Lukoshkova Elena V., Tarasova Olga S., Schubert Rudolf. TASK‐1 channel blockade by AVE1231 increases vasocontractile responses and BP in 1‐ to 2‐week‐old but not adult rats. British Journal of Pharmacology 2020;177(22):5148-5162. https://doi.org/10.1111/bph.15249
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Ma Dongyu, Gaynullina Dina, Schmidt Nadine, Mladenov Mitko, Schubert Rudolf. The functional availability of arterial Kv7 channels is suppressed considerably by large-conductance calcium-activated potassium channels in 2- to 3-month old but not in 10- to 15-day old rats. Frontiers in Physiology 2020;11:597395. https://doi.org/10.3389/fphys.2020.597395
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Zavaritskaya Olga, Dudem Srikanth, Ma Dongyu, Rabab Kaneez E., Albrecht Sarah, Tsvetkov Dmitry, Kassmann Mario, Thornbury Keith, Mladenov Mitko, Kammermeier Claire, Sergeant Gerard, Mullins Nicholas, Wouappi Ornella, Wurm Hannah, Kannt Aimo, Gollasch Maik, Hollywood Mark A., Schubert Rudolf. Vasodilation of rat skeletal muscle arteries by the novel BK channel opener GoSlo is mediated by the simultaneous activation of BK and Kv7 channels. British Journal of Pharmacology 2020;177(5):1164-1186. https://doi.org/10.1111/bph.14910
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2019

Akanji Oluwadamilola, Weinzierl Nina, Schubert Rudolf, Schilling Lothar. Acid sensing ion channels in rat cerebral arteries: Probing the expression pattern and vasomotor activity. Life Sciences 2019;227:193-200. https://doi.org/10.1016/j.lfs.2019.04.054
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Eckel Julia, Zavaritskaya Olga, Schüttpelz-Brauns Katrin, Schubert Rudolf. An explorative vs. traditional practical course: how to inspire scientific thinking in medical students. Advances in Physiology Education 2019;43(3):350-354. https://doi.org/10.1152/advan.00120.2018
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Gaynullina Dina, Schubert Rudolf, Tarasova Olga. Changes in endothelial nitric oxide production in systemic vessels during early ontogenesis—a key mechanism for the perinatal adaptation of the circulatory system. International Journal of Molecular Sciences 2019;20(6):1421. https://doi.org/10.3390/ijms20061421
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Tsvetkov Dmitry, Kolpakov Evgeniy, Kassmann Mario, Schubert Rudolf, Gollasch Maik. Distinguishing between biological and technical replicates in hypertension research on isolated arteries. Frontiers in Medicine 2019;6:126. https://doi.org/10.3389/fmed.2019.00126
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Shvetsova Anastasia A., Gaynullina Dina K., Tarasova Olga S., Schubert Rudolf. Negative feedback regulation of vasocontraction by potassium channels in 10- to 15-day-old rats: dominating role of Kv7 channels. Acta Physiologica 2019;225(2):e13176. https://doi.org/10.1111/apha.13176
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2018

Li Youhai, Lapina Natalia, Weinzierl Nina, Bonde Lisbeth, Boedtkjer Ebbe, Schubert Rudolf, Moshage Han, Wohlfart Paulus, Schilling Lothar. A novel method to isolate retinal and brain microvessels from individual rats: Microscopic and molecular biological characterization and application in hyperglycemic animals. Vascular Pharmacology 2018;110:24-30. https://doi.org/10.1016/j.vph.2018.07.001
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Mochalov S. V., Tarasova N. V., Kudryashova T. V., Gaynullina D. K., Kalenchuk V. U., Borovik A. S., Vorotnikov A. V., Tarasova O. S., Schubert Rudolf. Higher Ca2+-sensitivity of arterial contraction in 1-week-old rats is due to a greater Rho-kinase activity. Acta Physiologica 2018;223(3):e13044. https://doi.org/10.1111/apha.13044
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Braun Diana, Zollbrecht Christa, Dietze Stefanie, Schubert Rudolf, Golz Stefan, Summer Holger, Persson Pontus B., Carlström Mattias, Ludwig Marion, Patzak Andreas. Hypoxia/Reoxygenation of Rat Renal Arteries Impairs Vasorelaxation via Modulation of Endothelium-Independent sGC/cGMP/PKG Signaling. Frontiers in Physiology 2018;9(480). https://doi.org/10.3389/fphys.2018.00480
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Gollasch Maik, Welsh Donald G., Schubert Rudolf. Perivascular adipose tissue and the dynamic regulation of Kv 7 and Kir channels: Implications for resistant hypertension. Microcirculation 2018;25(1):e12434. https://doi.org/10.1111/micc.12434
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Krämer Bernhard K, Mang Johannes F, Schubert Rudolf. The Effect of Microgravity on Central Aortic Blood Pressure. American Journal of Hypertension 2018;31(11):1180-1182. https://doi.org/10.1093/ajh/hpy133
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Schmid Johannes, Müller Bettina, Heppeler David, Gaynullina Dina, Kassmann Mario, Gagov Hristo, Mladenov Mitko, Gollasch Maik, Schubert Rudolf. The Unexpected Role of Calcium‐Activated Potassium Channels: Limitation of NO‐Induced Arterial Relaxation. Journal of the American Heart Association 2018;7(7). https://doi.org/10.1161/jaha.117.007808
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Lubomirov L. T., Papadopoulos S., Filipova D., Baransi Sufian, Todorović D., Lake P., Metzler D., Hilsdorf S., Schubert Rudolf, Schroeter M. M., Pfitzer G.. The involvement of phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and MYPT1 isoform expression in NO/cGMP mediated differential vasoregulation of cerebral arteries compared to systemic arteries. Acta Physiologica 2018;224(1):e13079. https://doi.org/10.1111/apha.13079
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Schubert Rudolf. The second life of ion transporters as signal transducers. Acta Physiologica 2018;224(1):e13155. https://doi.org/10.1111/apha.13155
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2017

Liu Z. Z., Mathia S., Pahlitzsch T., Wennysia I. C., Persson P. B., Lai E. Y., Högner A., Xu M. Z., Schubert Rudolf, Rosenberger C., Patzak A.. Myoglobin facilitates angiotensin II-induced constriction of renal afferent arterioles. American Journal of Physiology-Renal Physiology 2017;312(5):F908-F916. https://doi.org/10.1152/ajprenal.00394.2016
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Gagov Hristo, Emilova Radoslava, Dimitrova Daniela, Mladenov Mitko, Schubert Rudolf. Perivascular adipose tissue as regulator of the force of artery contractions in health and disease. BioDiscovery 2017;20:e19831. https://doi.org/10.3897/biodiscovery.20.e19831
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Seker F., Hesser J., Brockmann M. A., Neumaier-Probst E., Groden C., Schubert Rudolf, Brockmann C.. Pharmacokinetic modeling of Intra-arterial nimodipine therapy for subarachnoid hemorrhage-related cerebral vasospasm. Clinical Neuroradiology 2017;27(2):199-203. https://doi.org/10.1007/s00062-015-0464-1
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Schubert Rudolf. Relaxation and contraction rates: underestimated parameters of vascular contractility?. Acta Physiologica 2017;219(1):9-10. https://doi.org/10.1111/apha.12810
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Zavaritskaya Olga, Lubomirov Lubomir T., Altay Serdar, Schubert Rudolf. Src tyrosine kinases contribute to serotonin-mediated contraction by regulating calcium-dependent pathways in rat skeletal muscle arteries. Pflügers Archiv - European Journal of Physiology 2017;469(5-6):767-777. https://doi.org/10.1007/s00424-017-1949-3
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2016

Grozdanova Radslava Emilova, Dimitrova Daniela, Mladenov Mitko, Hadzi-Petrushev Nikola, Daneva Teodora, Padeshki Plamen, Schubert Rudolf, Chichova Mariela, Lubomirov L., Simeonovska-Nokolova Daniela, Gagov Hristo. Diabetes converts arterial regulation by perivascular adipose tissue from relaxation into H(2)O(2)-mediated contraction. Physiological Research / Academia Scientiarum Bohemoslovaca 2016;65(5):799-807. https://doi.org/10.33549/physiolres.933037
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Heumann Philipp, Koenen Anna, Zavaritskaya Olga, Schütze Konrad, Ramm André, Schlüter Torsten, Steinbach Antje, Rettig Rainer, Schubert Rudolf, Grisk Olaf. Sympathetic denervation facilitates L-type Ca2+ channel activation in renal but not in mesenteric resistance arteries. Journal of Hypertension 2016;34(4):692-703. https://doi.org/10.1097/HJH.0000000000000856
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Tsvetkov Dmitry, Tano Jean-Yves, Kassmann Mario, Wang Ning, Schubert Rudolf, Gollasch Maik. The Role of DPO-1 and XE991-Sensitive Potassium Channels in Perivascular Adipose Tissue-Mediated Regulation of Vascular Tone. Frontiers in Physiology 2016;7. https://doi.org/10.3389/fphys.2016.00335
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2015

Gaynullina Dina, Dweep Harsh, Gloe Torsten, Tarasova Olga S., Sticht Carsten, Gretz Norbert, Schubert Rudolf. Alteration of mRNA and microRNA expression profiles in rat muscular type vasculature in early postnatal development. Scientific Reports 2015;5(1). https://doi.org/10.1038/srep11106
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Schubert Rudolf. Are microRNAs opening up a new world of regulation?. Acta Physiologica 2015;215(3):130-132. https://doi.org/10.1111/apha.12564
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Emilova Radoslava, Dimitrova Daniela, Mladenov Mitko, Daneva Teodora, Schubert Rudolf, Gagov Hristo. Cystathionine gamma-lyase of perivascular adipose tissue with reversed regulatory effect in diabetic rat artery. Biotechnology & Biotechnological Equipment 2015;29(1):147-151. https://doi.org/10.1080/13102818.2014.991565
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2014

Puzdrova V. A., Kudryashova T. V., Gaynullina D. K., Mochalov S. V., Aalkjaer C., Nilsson H., Vorotnikov A. V., Schubert Rudolf, Tarasova O. S.. Trophic action of sympathetic nerves reduces arterial smooth muscle Ca2+ sensitivity during early post-natal development in rats. Acta Physiologica 2014;212(2):128-141. https://doi.org/10.1111/apha.12331
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2013

Lidington Darcy, Schubert Rudolf, Bolz Steffen-Sebastian. Capitalizing on diversity: an integrative approach towards the multiplicity of cellular mechanisms underlying myogenic responsiveness. Cardiovascular Research 2013;97(3):404-412. https://doi.org/10.1093/cvr/cvs345
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Seker Fatih, Hesser Jürgen, Neumaier-Probst Eva, Groden Christoph, Brockmann Marc A., Schubert Rudolf, Brockmann Carolin. Dose–response relationship of locally applied nimodipine in an ex vivo model of cerebral vasospasm. Neuroradiology 2013;55(1):71-76. https://doi.org/10.1007/s00234-012-1079-8
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Gaynullina Dina, Lubomirov Lubomir T., Sofronova Svetlana I., Kalenchuk Vyacheslav U., Gloe Torsten, Pfitzer Gabriele, Tarasova Olga S., Schubert Rudolf. Functional remodelling of arterial endothelium during early postnatal development in rats. Cardiovascular Research 2013;99(4):612-621. https://doi.org/10.1093/cvr/cvt138
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Sazdova Iliyana V., Ilieva Bilyana M., Shkodrova Milena, Milusheva Alexandra, Chichova Mariela, Schubert Rudolf, Fülüp Ferenc, Lubomirov Lubomir T., Gagov Hristo S.. Obestatin Signalling in Excised Frog Heart. Proceeding of the Bulgarian Academy of Sciences 2013;66(6):847-856. https://doi.org/10.7546/cr-2013-66-6-13101331-10
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Iozzi D., Schubert Rudolf, Kalenchuk V. U., Neri A., Sgaragli G., Fusi F., Saponara S.. Quercetin relaxes rat tail main artery partlyviaa PKG-mediated stimulation of KCa1.1 channels. Acta Physiologica 2013;208(4):329-339. https://doi.org/10.1111/apha.12083
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Zavaritskaya Olga, Zhuravleva Nadezda, Schleifenbaum Johanna, Gloe Torsten, Devermann Lena, Kluge Reinhart, Mladenov Mitko, Frey Manfred, Gagov Hristo, Fésüs Gabor, Gollasch Maik, Schubert Rudolf. Role of KCNQ Channels in Skeletal Muscle Arteries and Periadventitial Vascular Dysfunction. Hypertension 2013;61(1):151-159. https://doi.org/10.1161/hypertensionaha.112.197566
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Abramochkin Denis V., Kuzmin Vladislav S., Mitrochin Vadim M., Kalugin Leonid, Dvorzhak Anton, Makarenko Ekaterina Y., Schubert Rudolf, Kamkin Andre. TNF-α provokes electrical abnormalities in rat atrial myocardium via a NO-dependent mechanism. Pflügers Archiv - European Journal of Physiology 2013;465(12):1741-1752. https://doi.org/10.1007/s00424-013-1320-2
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2012

Climent Belén, Schubert Rudolf, Stankevicius Edgaras, García-Sacristán Albino, Simonsen Ulf, Rivera Luis. Large conductance Ca2+-activated K+ channels modulate endothelial cell outward currents and nitric oxide release in the intact rat superior mesenteric artery. Biochemical and Biophysical Research Communications 2012;417(3):1007-1013. https://doi.org/10.1016/j.bbrc.2011.12.076
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2010

Schubert Rudolf, Sazdova Ilyana V., Ilieva Bilyana M., Shkodrova Milena, Fulop Ferenc, Gagov Hristo. Mechanism of the positive inotropic effect of dimethyl sulfoxide at low doses in excised frog heart preparations. Comptes rendus de l'Académie bulgare des sciences: sciences mathématiques et naturelles 2010;63(5):699-708.
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Schubert Rudolf, Leuner Kristina, Heiser J.H., Derksen S., Mladenov Mitko, Fehske C:J:, Gollasch M., Schneider G., Harteneck C., Müller W.E., Chatterjee Shyam Sunder. Simple 2,4-Diacylphloroglucinols as Classic Transient Receptor Potential-6 Activators-Identification of a Novel Pharmacophore. Molecular pharmacology 2010;77(3):368-377. https://doi.org/10.1124/mol.109.057513
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Schleifenbaum Johanna, Köhn Carolin, Voblova Nedzda, Dubrovska Galyna, Zavarirskaya Olga, Gloe Torsten, Crean Christopher S., Luft Friedrich C., Huang Yu, Schubert Rudolf, Gollasch Maik. Systemic peripheral artery relaxation by KCNQ channel openers and hydrogen sulfide. Journal of Hypertension 2010;28(9):1875-1882. https://doi.org/10.1097/HJH.0b013e32833c20d5
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Cooper T. K., Zhong Q., Krawczyk M., Tae H-J., Müller G. A., Schubert Rudolf, Myers L. A., Dietz H. C., Talan M. I., Briest W.. The haploinsufficient Col3a1 mouse as a model for vascular Ehlers-Danlos syndrome. Veterinary Pathology 2010;47(6):1028-1039. https://doi.org/10.1177/0300985810374842
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2009

Kirschstein Timo, Dammann Fabian, Klostermann Jenny, Rehberg Mirko, Tokay Tursonjan, Schubert Rudolf, Köhling Rüdiger. Dopamine induces contraction in the proximal, but relaxation in the distal rat isolated small intestine. Neuroscience Letters 2009;465(1):21-26. https://doi.org/10.1016/j.neulet.2009.08.080
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Anschütz Stephanie, Schubert Rudolf. Modulation of the myogenic response by neurogenic influences in rat small arteries. British Journal of Pharmacology 2009;146(2):226-233. https://doi.org/10.1038/sj.bjp.0706323
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Sazdova Iliyana, Ilieva Bilyana, Minkov Ignat, Schubert Rudolf, Gagov Hristo. Obestatin as contractile mediator of excised frog heart. Open Life Sciences 2009;4(3):327-334. https://doi.org/10.2478/s11535-009-0023-3
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Tarasova O.S., Puzdrova V.A., Tarasova N.V., Mochalov S.V., Vorotnikov A.V., Schubert Rudolf. P3.11 Rapid contractile phenotype of vascular smooth muscle is controlled by trophic influence of sympathetic nerves. Autonomic Neuroscience 2009;149(1-2):86-87. https://doi.org/10.1016/j.autneu.2009.05.159
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2008

Mladenov Mitko, Hristov Kiril, Dimitriova D. Z., Schubert Rudolf, Lubomirov L. T., Gjorgoski Icko, Duridanova D. B., Gagov Hristo. Ghrelin signalling in guinea-pig femoral artery smooth muscle cells. Acta Physiologica 2008;194(3):195-206. https://doi.org/10.1111/j.1748-1716.2008.01880.x
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2007

Ivanova Iliyana V., Schubert Rudolf, Duridanova Dessislava B., Bolton Thomas B., Lubomirov Lubomir T., Gagov Hristo S.. Cocaine- and amphetamine-regulated transcript (CART) peptide as anin vivoregulator of cardiac function inRana ridibundafrog. Experimental Physiology 2007;92(6):1037-1046. https://doi.org/10.1113/expphysiol.2007.038935
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Schubert Rudolf, Lidington D., Bolz S.. The emerging role of Ca2+ sensitivity regulation in promoting myogenic vasoconstriction. Cardiovascular Research 2007;77(1):8-18. https://doi.org/10.1016/j.cardiores.2007.07.018
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2006

Köhler Ralf, Heyken Willm-Thomas, Heinau Philipp, Schubert Rudolf, Si Han, Kacik Michael, Busch Christoph, Grgic Ivica, Maier Tanja, Hoyer Joachim. Evidence for a Functional Role of Endothelial Transient Receptor Potential V4 in Shear Stress–Induced Vasodilatation. Arteriosclerosis, Thrombosis, and Vascular Biology 2006;26(7):1495-1502. https://doi.org/10.1161/01.atv.0000225698.36212.6a
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Si Han, Heyken Willm-Thomas, Wölfle Stephanie E., Tysiac Marcin, Schubert Rudolf, Grgic Ivica, Vilianovich Larisa, Giebing Günter, Maier Tanja, Gross Volkmar, Bader Michael, de Wit Cor, Hoyer Joachim, Köhler Ralf. Impaired Endothelium-Derived Hyperpolarizing Factor-Mediated Dilations and Increased Blood Pressure in Mice Deficient of the Intermediate-Conductance Ca2+-Activated K+Channel. Circulation Research 2006;99(5):537-544. https://doi.org/10.1161/01.res.0000238377.08219.0c
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Rust Marco B., Faulhaber Jörg, Budack Mareike K., Pfeffer Carsten, Maritzen Tanja, Didié Michael, Beck Franz-Xaver, Boettger Thomas, Schubert Rudolf, Ehmke Heimo, Jentsch Thomas J., Hübner Christian A.. Neurogenic Mechanisms Contribute to Hypertension in Mice With Disruption of the K-Cl Cotransporter KCC3. Circulation Research 2006;98(4):549-556. https://doi.org/10.1161/01.res.0000204449.83861.22
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Pfitzer G., Lubomirov L. T., Reimann K., Gagov H., Schubert Rudolf. Regulation of the crossbridge cycle in vascular smooth muscle by cAMP signalling. Journal of Muscle Research and Cell Motility 2006;27(5-7):445-454. https://doi.org/10.1007/s10974-006-9097-y
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Schubert Rudolf. Sphingosine-1-phosphate in the circulatory system: Cause and therapeutic target for vascular dysfunction?. Cardiovascular Research 2006;70(1):9-11. https://doi.org/10.1016/j.cardiores.2006.02.012
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Lubomirov L. T., Schubert Rudolf, Gagov Hristo, Duridanova D. B., Pfitzer G.. Urocortin increases the intracellular cAMP concentration and thus decreases the degree of phosphorylation of MYPT1 and increases the myosin phosphatase activity. Biophysics 2006;51(5):679-686. https://doi.org/10.1134/s0006350906050010
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Lubomirov Lubomir T., Reimann Katrin, Metzler Doris, Hasse Veronika, Stehle Robert, Ito Masaaki, Hartshorne David J., Gagov Hristo, Pfitzer Gabriele, Schubert Rudolf. Urocortin-Induced Decrease in Ca2+ Sensitivity of contraction in Mouse Tail Arteries is Attributable to cAMP-Dependent Dephosphorylation of MYPT1 and Activation of Myosin Light Chain Phosphatase. Circulation Research 2006;98(9):1159-1167. https://doi.org/10.1161/01.res.0000219904.43852.3e
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2005

Ruth Peter, Bucurenciu Iancu, Zhao Hong, Zhou Xiao-Bo, Sausbier Ulrike, Arntz Claudia, Feil Susi, Essin Kyrill, Feil Robert, Hofmann Franz, Knaus Hans-Günther, Shipston Michael J, Storm Johan, Korth Michael, Schubert Rudolf, Gollasch Maik, Sausbier Matthias. Analysis of BKCa channel deficient mice. BMC Pharmacology 2005;5(Suppl 1):S39. https://doi.org/10.1186/1471-2210-5-s1-s39
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Sausbier Matthias, Arntz Claudia, Bucurenciu Iancu, Zhao Hong, Zhou Xiao-Bo, Sausbier Ulrike, Feil Susanne, Kamm Simone, Essin Kyrill, Sailer Claudia A., Abdullah Usamah, Krippeit-Drews Peter, Feil Robert, Hofmann Franz, Knaus Hans-Günther, Kenyon Chris, Shipston Michael J., Storm Johan F., Neuhuber Winfried, Korth Michael, Schubert Rudolf, Gollasch Maik, Ruth Peter. Elevated Blood Pressure Linked to Primary Hyperaldosteronism and Impaired Vasodilation in BK Channel–Deficient Mice. Circulation 2005;112(1):60-68. https://doi.org/10.1161/01.cir.0000156448.74296.fe
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Sausbier Matthias, Zhao Hong, Schubert Rudolf, Sausbier Ulrike, Abdullah U, Huber Andrea, Feil Susi, Feil Robert, Hofmann Franz, Neuhuber Winfried, Allescher Hans-Dieter, Ruth Peter. Enhanced vascular cGMP/cGK I signaling and hypotonia in cysteine-rich-protein 2-deficient mice. BMC Pharmacology 2005;5(Suppl 1):P48. https://doi.org/10.1186/1471-2210-5-s1-p48
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Schubert Rudolf. Non-capacitative calcium entry—Extension of the possibilities for calcium entry in vascular tissue. Cardiovascular Research 2005;68(1):5-7. https://doi.org/10.1016/j.cardiores.2005.07.018
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2004

Schubert Rudolf, Krien Ulrike, Wulfsen Iris, Schiemann Dorrit, Lehmann Gernot, Ulfig Norbert, Veh Ruediger W., Schwarz Jürgen R., Gago Hristo. Nitric Oxide Donor Sodium Nitroprusside Dilates Rat Small Arteries by Activation of Inward Rectifier Potassium Channels. Hypertension 2004;43(4):891-896. https://doi.org/10.1161/01.hyp.0000121882.42731.6b
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2002

Schubert Rudolf, Kalentchuk Vjatscheslav U., Krien Ulrike. Rho kinase inhibition partly weakens myogenic reactivity in rat small arteries by changing calcium sensitivity. American Journal of Physiology-Heart and Circulatory Physiology 2002;283(6):H2288-H2295. https://doi.org/10.1152/ajpheart.00549.2002
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2001

Schubert Rudolf, Nelson Mark T.. Protein kinases: tuners of the BKCa channel in smooth muscle. Trends in Pharmacological Sciences 2001;22(10):505-512. https://doi.org/10.1016/s0165-6147(00)01775-2
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Schubert Rudolf, Krien Ulrike, Gagov Hristo. Protons Inhibit the BKCa Channel of Rat Small Artery Smooth Muscle Cells. Journal of Vascular Research 2001;38(1):30-38. https://doi.org/10.1159/000051027
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Lubomirov Lubomir, Gagov Hristo, Petkova-Kirova Polina, Duridanova Dessislava, Kalentchuk Vjatscheslav U., Schubert Rudolf. Urocortin relaxes rat tail arteries by a PKA-mediated reduction of the sensitivity of the contractile apparatus for calcium. British Journal of Pharmacology 2001;134(7):1564-1570. https://doi.org/10.1038/sj.bjp.0704418
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Strauss Ulf, Herbrik Martin, Mix Eilhard, Schubert Rudolf, Rolfs Arndt. Whole-cell patch-clamp: true perforated or spontaneous conventional recordings?. Pflügers Archiv: European Journal of Physiology 2001;442(4):634-638. https://doi.org/10.1007/s004240100578
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2000

Petkova-Kirova Polina, Gagov Hristo, Krien Ulrike, Duridanova Dessislava, Noack Thomas, Schubert Rudolf. 4-Aminopyridine affects rat arterial smooth muscle BKCacurrents by changing intracellular pH. British Journal of Pharmacology 2000;131(8):1643-1650. https://doi.org/10.1038/sj.bjp.0703742
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Sausbier Matthias, Schubert Rudolf, Voigt Viktor, Hirneiss Christoph, Pfeifer Alexander, Korth Michael, Kleppisch Thomas, Ruth Peter, Hofmann Franz. Mechanisms of NO/cGMP-Dependent Vasorelaxation. Circulation Research 2000;87(9):825-830. https://doi.org/10.1161/01.res.87.9.825
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1999

Schubert Rudolf, Noack Thomas, Serebryakov Vladimir N.. Protein kinase C reduces the KCa current of rat tail artery smooth muscle cells. American Journal of Physiology-Cell Physiology 1999;276(3):C648-C658. https://doi.org/10.1152/ajpcell.1999.276.3.c648
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Schubert Rudolf, Mulvany Michael J.. The myogenic response: established facts and attractive hypotheses. Clinical Science 1999;96(4):313-326. https://doi.org/10.1042/cs19980403
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Schubert Rudolf, Lehmann Gernot, Serebryakov Vladimir N., Mewes Hartmut, Hopp Hans-Heinrich. cAMP-dependent protein kinase is in an active state in rat small arteries possessing a myogenic tone. American Journal of Physiology: Heart and Circulatory Physiology 1999;277(3):H1145-H1155. https://doi.org/10.1152/ajpheart.1999.277.3.h1145
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1998

Schubert Rudolf, Strauss Ulf, Jung S., Mix E.. K+ currents of encephalitogenic memory T cells decrease with encephalitogenicity while interleukin-2 (IL-2) receptor expression remains stable during IL-2 dependent cell expansion. Receptors and Channels 1998;6(1):73-87.
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Stimulation of vascular smooth muscle cell K-Ca current by iloprost is smaller in SHR than in WKY rats. Journal of Vascular Research 1998;35(3):201-202. https://doi.org/10.1159/000025584
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Joint Meeting of the Benelux Society for Microcirculation

1997

Schubert Rudolf, Serebryakov V. N., Mewes H., Hopp H. H.. Iloprost dilates rat small arteries: role of K(ATP)- and K(Ca)-channel activation by cAMP-dependent protein kinase. American Journal of Physiology: Heart and Circulatory Physiology 1997;272(3):H1147-H1156. https://doi.org/10.1152/ajpheart.1997.272.3.h1147
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Wesselman J. P., Schubert Rudolf, VanBavel E. D., Nilsson H., Mulvany M. J.. KCa-channel blockade prevents sustained pressure-induced depolarization in rat mesenteric small arteries. American Journal of Physiology: Heart and Circulatory Physiology 1997;272(5):H2241-H2249. https://doi.org/10.1152/ajpheart.1997.272.5.h2241
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Schubert Rudolf, Serebryankov V. N.. The iloprost-induced increase of calcium-activated potassium currents is smaller in hypertensive compared to normotensive rat tail artery smooth muscle cells. Hypertension 1997;30(4):71.
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1996

Fischer Jörg-Gerald, Mewes Hartmut, Hopp Hans-Heinrich, Schubert Rudolf. Analysis of pressurized resistance vessel diameter changes with a low cost digital image processing device. Computer Methods and Programs in Biomedicine 1996;50(1):23-30. https://doi.org/10.1016/0169-2607(96)01726-9
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Strauß Ulf, Wittstock Ute, Schubert Rudolf, Teuscher Eberhard, Jung Stefan, Mix Eilhard. Cicutoxin from Cicuta virosa: a New and Potent Potassium Channel Blocker in T Lymphocytes. Biochemical and Biophysical Research Communications 1996;219(2):332-336. https://doi.org/10.1006/bbrc.1996.0233
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Schubert Rudolf, Serebryakov V. N., Engel H., Hopp H. H.. Iloprost activates KCa channels of vascular smooth muscle cells: role of cAMP-dependent protein kinase. American Journal of Physiology: Cell Physiology 1996;271(4):C1203-C1211. https://doi.org/10.1152/ajpcell.1996.271.4.c1203
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Schubert Rudolf. Multiple Ligand-Ion Solutions: A Guide for Solution Preparation and Computer Program Understanding. Journal of Vascular Research 1996;33(1):86-98. https://doi.org/10.1159/000159136
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Schubert Rudolf, Wesselman Jos P. M., Nilsson Holger, Mulvany Michael J.. Noradrenaline-induced depolarization is smaller in isobaric compared to isometric preparations of rat mesenteric small arteries. Pflügers Archiv: European Journal of Physiology 1996;431(5):794-796. https://doi.org/10.1007/bf02253846
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Physiologie

AG Schubert

Projekte

Regulation and function of vascular smooth muscle potassium channels

References

Functional remodeling of vascular smooth muscle during early postnatal development

References

Mechanotransduction mechanisms in vascular smooth muscle cells

References

Mechanisms of calcium sensitivity regulation

References

Publikationen

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